In the construction of a DNA library, bacterial colonies that grow on agar plates containing antibiotic can be described as:
A) arising from one individual bacterium that contained a recombinant plasmid
B) arising from many individual bacteria, only some of which contain a recombinant plasmid
C) arising from one individual bacterium that does not contain a recombinant plasmid
D) arising from wild-type bacteria
E) containing a single bacterium, which contains a recombinant plasmid

Question

Quizplus · Accepted Answer

Bacterial colonies that grow on agar plates containing antibiotic are typically the result of transformation with a plasmid that contains an antibiotic resistance gene. Each colony arises from a single bacterium that took up a recombinant plasmid, allowing it to grow in the presence of the antibiotic.

In the Construction of a DNA Library, Bacterial Colonies That