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Match the Sequence of the Steps for the Southern Blot

Question 98

Matching

Match the sequence of the steps for the Southern Blot assay.

Premises:

Responses:

Step 1

The radiograph shows the hybridized DNA fragments.

Visualize the DNA fragments using UV light.

The blotting buffer transfers DNA fragments to DNA binding filter.

Place the filter and radioactively labeled probe together in the sealed bag.

Load the DNA fragments on the gel and separate by electrophoresis.

Premises:

Step 1

Responses:

The radiograph shows the hybridized DNA fragments.

Visualize the DNA fragments using UV light.

The blotting buffer transfers DNA fragments to DNA binding filter.

Place the filter and radioactively labeled probe together in the sealed bag.

Load the DNA fragments on the gel and separate by electrophoresis.

Correct Answer:

Premises:

Responses:

The radiograph shows the hybridized DNA fragments.

Visualize the DNA fragments using UV light.

The blotting buffer transfers DNA fragments to DNA binding filter.

Place the filter and radioactively labeled probe together in the sealed bag.

Load the DNA fragments on the gel and separate by electrophoresis.

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