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Match the Sequence of the Steps for the Southern Blot

Question 81

Matching

Match the sequence of the steps for the Southern Blot assay.

Premises:

Responses:

Step 4

Load the DNA fragments on the gel and separate by electrophoresis.

Step 1

Place the filter and radioactively labeled probe together in the sealed bag.

Step 2

Visualize the DNA fragments using UV light.

Step 3

The blotting buffer transfers DNA fragments to DNA binding filter.

Step 5

The radiograph shows the hybridized DNA fragments.

Premises:

Step 4

Step 1

Step 2

Step 3

Step 5

Responses:

Load the DNA fragments on the gel and separate by electrophoresis.

Place the filter and radioactively labeled probe together in the sealed bag.

Visualize the DNA fragments using UV light.

The blotting buffer transfers DNA fragments to DNA binding filter.

The radiograph shows the hybridized DNA fragments.

Correct Answer:

Premises:

Responses:

Step 4

Step 1

Step 2

Step 3

Step 5

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