Question 1

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once.
-Requires washing the cells several times before the addition of antihuman globulin reagent
A)Indirect antiglobulin test
B)Direct antiglobulin test
C)Both the direct and indirect antiglobulin test

Accepted Answer

Both the direct and indirect antiglobulin tests require washing the cells several times before the addition of antihuman globulin reagent to remove any unbound antibodies or proteins that could interfere with the test results.

Question 2

MATCHING
Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent used to detect the presence of red cell antibodies
A)Panel cells
B)Screening cells
C)A1 and B cells
D)ABO antisera
E)Lectins

Accepted Answer

Screening cells are used to detect the presence of red cell antibodies in a patient's serum or plasma. A group of reagent red blood cells that are known not to have certain antigens are incubated with the patient's serum or plasma. If antibodies are present in the serum or plasma, they will react with the corresponding antigens on the screening cells. The presence of a reaction indicates the need for further testing to identify the specific antibody present.

Question 3

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once.
-Detects IgG or complement-coated red cells
A)Indirect antiglobulin test
B)Direct antiglobulin test
C)Both the direct and indirect antiglobulin test

Accepted Answer

Both the direct and indirect antiglobulin test can be used to detect IgG or complement-coated red cells. The direct test is used to detect antibodies already attached to the surface of red blood cells in the patient's body, while the indirect test is used to detect antibodies present in the serum against red blood cells. Both can identify the presence of IgG or complement on red cells.

Question 4

What immunoglobulin class reacts best by antiglobulin testing?
A) IgM
B) IgA
C) IgE
D) IgG

Accepted Answer

The antiglobulin test detects IgG antibodies on red cells.

Question 5

Why is polyethylene glycol reagent added to the screen or panel?
A) Enhance detection of IgM antibodies.
B) Eliminate the reactivity of certain antigens.
C) Increase the avidity of IgG antibodies.
D) Eliminate the need for washing in the indirect antiglobulin test.

Accepted Answer

Polyethylene glycol (PEG)concentrates antibodies and increases the rate of antibody uptake,increasing the avidity of IgG antibody reactions.

Question 6

Which of the following red cell antigens do proteolytic enzymes destroy?
A) Rh system antigens
B) Antigens Fyᵃ and Fyᵇ in the Duffy system
C) Antigens in the Kidd system
D) Lewis system antigens

Accepted Answer

Proteolytic enzymes,such as ficin,will destroy some antigens on red cells such as Fyᵃ and Fyᵇ,M,N,and S.

Question 7

What is the purpose of adding antibody-sensitized red cells following the antiglobulin test?
A) Ensure a weak antibody reaction was not missed
B) Confirm positive reactions
C) Check that the wash procedure was sufficient to remove unbound antibodies
D) Check that sufficient incubation took place

Accepted Answer

Antibody-sensitized red cells (check cells)are IgG-coated cells that will detect unbound antihuman globulin following proper washing techniques.

Question 8

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once.
-Incubation step is not necessary
A)Indirect antiglobulin test
B)Direct antiglobulin test
C)Both the direct and indirect antiglobulin test

Accepted Answer

The direct antiglobulin test (DAT) does not require an incubation step as it involves testing for antibodies already bound to red blood cells in the patient's circulation.

Question 9

MATCHING
Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent derived from plants used to distinguish group A₁ from group A₂ red cells
A)Panel cells
B)Screening cells
C)A1 and B cells
D)ABO antisera
E)Lectins

Accepted Answer

Lectins are reagents derived from plants that are used to distinguish between group A1 and A2 red blood cells. Group A2 red blood cells contain a weaker form of the A antigen compared to group A1, and thus will not agglutinate with anti-A1 antibodies but will agglutinate with certain lectins such as Dolichos biflorus. This distinction is important when selecting blood for transfusion, as individuals with group A2 blood can potentially develop antibodies to the A1 antigen if given a transfusion of group A1 blood.

Question 10

Rouleaux is a false-positive reaction caused by elevated serum protein levels.Which of the following tests would not likely be affected by an elevated protein level?
A) Immediate-spin antibody screen
B) Direct antiglobulin test
C) Reverse typing in the ABO test
D) ABO forward typing

Accepted Answer

Rouleaux are caused by an elevated protein level or IV solutions and cause cells to appear agglutinated.A procedure involving washing,such as the direct or indirect antiglobulin test,would not be affected by this because saline would eliminate the excess proteins.

Question 11

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once.
-Tests for certain clinical conditions such as hemolytic disease of the newborn and autoimmune hemolytic anemia
A)Indirect antiglobulin test
B)Direct antiglobulin test
C)Both the direct and indirect antiglobulin test

Accepted Answer

The Direct Antiglobulin Test (DAT) is used to detect antibodies or complement proteins attached to the surface of red blood cells, which is useful in diagnosing conditions like hemolytic disease of the newborn and autoimmune hemolytic anemia.

Question 12

MATCHING
Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent used in the identification of ABO antibodies
A)Panel cells
B)Screening cells
C)A1 and B cells
D)ABO antisera
E)Lectins

Accepted Answer

A1 and B cells are used in the identification of ABO antibodies because they can help differentiate between the ABO blood groups by reacting with the antibodies present in the patient's serum.

Question 13

MATCHING
Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent used to determine the ABO antigenic composition of a patient's red cells
A)Panel cells
B)Screening cells
C)A1 and B cells
D)ABO antisera
E)Lectins

Accepted Answer

ABO antisera are used to identify the ABO blood group antigens present on the surface of the patient's red blood cells.

Question 14

MATCHING
Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent used to identify the specificity of a red cell antibody
A)Panel cells
B)Screening cells
C)A1 and B cells
D)ABO antisera
E)Lectins

Accepted Answer

Panel cells are used to identify the specificity of a red cell antibody. They contain known antigens on their surface and are used to determine which specific antibody is present in a patient's serum.

Quiz 3: Blood Banking Reagents: Overview and Applications

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once. -Requires washing the cells several times before the addition of antihuman globulin reagent

MATCHING Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used to detect the presence of red cell antibodies

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once. -Detects IgG or complement-coated red cells

What immunoglobulin class reacts best by antiglobulin testing?

Why is polyethylene glycol reagent added to the screen or panel?

Which of the following red cell antigens do proteolytic enzymes destroy?

What is the purpose of adding antibody-sensitized red cells following the antiglobulin test?

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once. -Incubation step is not necessary

MATCHING Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent derived from plants used to distinguish group A₁ from group A₂ red cells

Rouleaux is a false-positive reaction caused by elevated serum protein levels.Which of the following tests would not likely be affected by an elevated protein level?

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once. -Tests for certain clinical conditions such as hemolytic disease of the newborn and autoimmune hemolytic anemia

MATCHING Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used in the identification of ABO antibodies

MATCHING Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used to determine the ABO antigenic composition of a patient's red cells

MATCHING Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used to identify the specificity of a red cell antibody