Indicate the correct order of steps for the production of monoclonal antibodies.
1) Hybridomas transferred to small wells and tested for antigen reactivity
2) B cells are harvested from the spleen of a mouse injected with antigen
3) Mixture of B cells, myeloma cells, and hybridomas are transferred to media that promotes only hybridoma survival
4) Long-lived hybridomas are grown in culture and their monoclonal antibodies collected for use
5) B cells mixed with multiple myeloma cells in special growth media to encourage hybridoma formation

Question

Quizplus · Accepted Answer

The correct order starts with harvesting B cells from the spleen of a mouse that has been injected with the antigen (2), followed by mixing these B cells with myeloma cells in a special growth media to encourage the formation of hybridomas (5). Next, the mixture, including B cells, myeloma cells, and the newly formed hybridomas, is transferred to a media that allows only the hybridomas to survive (3). The hybridomas are then transferred to small wells to test for antigen reactivity (1), and finally, the long-lived hybridomas are grown in culture, and their monoclonal antibodies are collected for use (4).

Indicate the Correct Order of Steps for the Production of Monoclonal