Which method revealed that there were many more RNAs transcribed than are accounted for by the protein-coding genes in human cells?
A) DNA microarray
B) Next-generation sequencing
C) RNA-seq
D) cDNA hybridization

Question

Quizplus · Accepted Answer

RNA-seq is the method that allows for high-throughput sequencing of all RNA molecules in a sample, identifying both coding and non-coding RNAs. This approach revealed that there are numerous non-coding RNAs transcribed from regions of the genome previously thought to be "junk DNA" and highlighted the importance of studying these poorly understood transcripts. DNA microarray and cDNA hybridization are both methods that can be used to measure the expression of specific genes, but they do not provide a comprehensive view of all RNA molecules in a sample. Next-generation sequencing can be used for RNA-seq, but is not more specific than RNA-seq.

Which Method Revealed That There Were Many More RNAs Transcribed