In tandem mass spectrometry the unique second phase is characterized by
A) the further isolation of the first phase samples by two-dimensional gel electrophoresis.
B) the purification of the first phase samples by column chromatography.
C) the addition of a collision based fragmentation step to the first phase sample to determine amino acid sequence.
D) running simultaneous samples to compare and confirm amino acid identity.

Question

Quizplus · Accepted Answer

In tandem mass spectrometry, the second phase involves performing collision-based fragmentation on the first phase sample to determine the amino acid sequence of the peptides detected. This fragmentation generates a set of fragment ions that can be used to identify the peptide and its sequence. Column chromatography and two-dimensional gel electrophoresis may be used in the first phase to isolate and purify the samples, and running simultaneous samples may be used to compare and confirm amino acid identity, but these techniques do not specifically characterize the unique second phase of tandem mass spectrometry.

In Tandem Mass Spectrometry the Unique Second Phase Is Characterized