The trypsin substrate binding site contains an aspartate residue, which is able to form an ionic bond with which amino acids in its polypeptide substrates?
A) Lysine or arginine
B) Glutamate or glutamine
C) Leucine or phenylalanine
D) Serine or threonine

Question

Quizplus · Accepted Answer

The negatively charged aspartate residue in trypsin's substrate binding site is attracted to the positively charged amino acids lysine or arginine, which are commonly found in the substrates that trypsin cleaves. This ionic interaction helps position the substrate for cleavage by trypsin's active site.

The Trypsin Substrate Binding Site Contains an Aspartate Residue, Which