If a growing DNA strand incorporates a ddNTP during a cycle sequencing reaction, the elongation…
A) Will continue to grow by incorporating nucleotides
B) Will continue to grow if the next nucleotide is a dNTP
C) Will terminate at that point in the elongation process
D) Will terminate when the next dNTP gets incorporated
E) Choice "a" & 'b' are correct

Question

Quizplus · Accepted Answer

The incorporation of ddNTP stops the elongation process because it lacks a 3'-OH group necessary for forming a phosphodiester bond with the next incoming nucleotide, resulting in chain termination at that point.

If a Growing DNA Strand Incorporates a DdNTP During a Cycle