Scientists at Cal Tech (Pasadena, Ca. and Applied Biosystems (Foster City, Ca.) improved upon the Sanger sequencing method. Which one of the following statements is not true.
A) Replacing the use of radioactive isotopes with fluorescent dyes
B) Detection of these dyes using UV-vis spectrophotometer
C) A machine developed by Applied Biosystems that was an automated sequencing instrument
D) Employed the Sanger approach with ddNTPs as terminating bases
E) Primers were labeled with fluorescent dyes (dye primer approach)

Question

Quizplus · Accepted Answer

The Sanger sequencing method improved by Cal Tech and Applied Biosystems used fluorescent dyes for detection, but the detection was not done using a UV-vis spectrophotometer. Instead, the machine developed by Applied Biosystems used a laser to excite the fluorescent dyes and a detector to measure the emission of light.