Which method(s) can be used to produce mice that lack brain serotonin throughout life, beginning with embryonic development?
A) Administering the tryptophan hydroxylase inhibitor PCPA
B) Creating knockout mice that lack 5-HT
C) Preventing the normal differentiation of cells that are destined to become serotonergic neurons
D) Both b and c

Question

Quizplus · Accepted Answer

Administering a tryptophan hydroxylase inhibitor (A) can temporarily reduce brain serotonin levels, but it does not completely eliminate serotonin nor prevent the development of serotonergic neurons. 
Creating knockout mice that lack 5-HT (B) is a feasible method to produce mice lacking brain serotonin throughout life, as 5-HT is a critical component in the synthesis of serotonin. 
Preventing the normal differentiation of cells that are destined to become serotonergic neurons (C) is also a viable method, as it would prevent the development of these neurons that synthesize and release serotonin. 
Therefore, D is the best choice because both B and C are valid methods.

Which Method(s) Can Be Used to Produce Mice That Lack