Which of the following is not part of the procedure and/or interpretation of radioligand binding?
A) Tissue is incubated with radioligand.
B) Specificity is measured by adding radioactive competing ligand.
C) Saturability is measured by adding increasing amounts of ligand.
D) Reversibility is measured by showing displacement of ligand by drug.

Question

Quizplus · Accepted Answer

Specificity in radioligand binding assays is typically measured by the ability of a non-radioactive (cold) competing ligand to displace the radioligand, not by adding a radioactive competing ligand. The use of a non-radioactive competitor helps to assess the specificity of the binding to the receptor of interest without introducing additional radioactivity that could complicate the interpretation.

Which of the Following Is Not Part of the Procedure