In immunocytochemistry,
A) tissue slices are cut and incubated with antigen in solution.
B) the antibody response is not very selective because it recognizes a class of similar proteins.
C) antibodies must initially be tested by injecting them into host animals.
D) cells making the neuropeptide hypocretin can be visualized via creating tagged antibodies to the neuropeptide.

Question

Quizplus · Accepted Answer

Immunocytochemistry involves using specific antibodies to visualize and identify specific molecules or cells within tissue samples. In this case, creating tagged antibodies to the neuropeptide hypocretin allows for visualization of cells that produce this neuropeptide. Choice A is incorrect because tissue slices are typically first fixed and then incubated with primary and secondary antibodies, not with antigen in solution. Choice B is incorrect because antibody response can be highly selective and specific to a particular protein of interest. Choice C is incorrect because antibodies can also be tested and validated using alternative techniques such as Western blotting or ELISA assays.

In Immunocytochemistry