Given the following data obtained from a GC-MS analysis using a 15m column::
Peak 1: Retention time 4.32 minutes; FWHM 16. seconds
Peak 2: Retention time 5.87 minutes; FWHM 19 seconds
What does the calculated resolution value, based on these two peaks, mean in terms of chromatographic appearance?

Question

Quizplus · Accepted Answer

The resolution between two peaks in chromatography is calculated using the formula: Resolution = 2(t2 - t1) / (W1 + W2), where t1 and t2 are the retention times of the two peaks, and W1 and W2 are the widths of the peaks at half height (FWHM). For the given data, the resolution calculation would indicate how well the peaks are separated. A resolution value between 1 and 1.5 generally indicates that peaks will overlap slightly, meaning they are not fully baseline resolved but are distinguishable from each other.

Given the Following Data Obtained from a GC-MS Analysis Using