The pH profile of an enzyme can help identify specific amino acids in the active site because:
A) all enzymes have a pH optimum
B) only the active site amino acids can detect changes in pH
C) acidic and basic amino acids are often involved in the active site and pH changes can change their ability to catalyze a reaction
D) the pH optimum is always the pI of the most critical amino acid in the active site

Question

Quizplus · Accepted Answer

Acidic and basic amino acids in the active site are sensitive to pH changes, which can affect their charge and thus their ability to participate in catalysis. This is why the pH profile of an enzyme can provide insights into the specific amino acids present in the active site.

The PH Profile of an Enzyme Can Help Identify Specific