Below Is a 2D Chromatogram That Shows the Separation of Five

Below is a 2D chromatogram that shows the separation of five amino acids. In this technique, the amino acid mixture is first separated by chromatography using a polar solvent system. Then the plate is rotated 90°, and the amino acids are further separated by electrophoresis. Identify the spots obtained from a mixture of Trp, Glu, Lys, Ile and Thr. A table with isoelectric points is included to aid in solving this problem.
Amino acid-pI value
Trp-5.9
Glu-3.2
Lys-9.7
Ile-6.0
Thr-5.6

A) 1=Glu, 2=Ile, 3=Lys, 4=Trp, 5=Thr
B) 1=Lys, 2=Thr, 3=Glu, 4=Trp, 5=Ile
C) 1=Glu, 2=Thr, 3=Lys, 4=Trp, 5=Ile
D) 1=Lys, 2=Ile, 3=Glu, 4=Trp, 5=Thr