Which amino acid side chains would need to be protected in SPPS?
I. hydroxyl groups of tyrosine and serine
II. carboxyl groups of glutamic acid and aspartic acid
III. amine group of lysine
IV. methyl group of alanine
V. phenyl group of phenylalanine

Question

Quizplus · Accepted Answer

In Solid Phase Peptide Synthesis (SPPS), side chain protection is necessary for amino acids that have reactive functional groups to prevent unwanted side reactions. Hydroxyl groups (in tyrosine and serine), carboxyl groups (in glutamic acid and aspartic acid), and amine groups (in lysine) are reactive and need protection. Methyl groups (in alanine) and phenyl groups (in phenylalanine) do not typically require protection because they are not reactive under the conditions used in SPPS.

Which Amino Acid Side Chains Would Need to Be Protected