Expression Vectors Are Engineered Fragments of DNA That Contain the Necessary

Expression vectors are engineered fragments of DNA that contain the necessary information, such as ______, for the cell to be able to express the protein at a high level.
I. cloning sites tailored to various restriction enzymes
II. promoters designed to work in the individual cell lines
III. an origin of replication
IV. visualization sites
V. antibiotics resistance marker gene

A) I
B) II
C) III
D) II, III, and V
E) I, II, and III