Mammalian protein expression systems __________.
I. sometimes use the strong viral promoter CMV
II. are used when the expressed proteins require post-translational modifications
III. require expensive cell culture reagents
IV. are easy to handle
V. are used for producing insulin analogs

Question

Quizplus · Accepted Answer

Mammalian protein expression systems often use the strong viral promoter CMV to drive high levels of protein expression. They are particularly used when the expressed proteins require specific post-translational modifications that are unique to mammalian cells, such as glycosylation. These systems do require expensive cell culture reagents, making them more costly compared to bacterial or yeast expression systems. They are not necessarily easy to handle due to the complexity and requirements of maintaining mammalian cell cultures. Mammalian systems are indeed used for producing complex proteins like insulin analogs that require specific mammalian post-translational modifications for proper function.

Mammalian Protein Expression Systems __________