Proteins expressed in bacterial cells ________.
I. fold properly if they contain several domains
II. have to be released from the cells during a cell lysis step
III. do not go through post-translational modifications
IV. are secreted into the growth media

Question

Quizplus · Accepted Answer

Proteins expressed in bacterial cells often do not undergo post-translational modifications like those in eukaryotic cells, because bacteria lack the cellular machinery for most of these modifications. Additionally, to isolate and purify the proteins expressed in bacterial cells, the cells typically need to be lysed to release the proteins, as they are not usually secreted into the growth media.

Proteins Expressed in Bacterial Cells ________