When using cassette mutagenesis, in order to insert an engineered cDNA sequence into a plasmid, a __________ is used to cut out the sequence that is no longer needed, and a __________ is used to insert the new mutant cassette into the plasmid.
A) restriction enzyme; ligase
B) clone; enzyme
C) catalase; polymerase
D) reductase; polymerase
E) all of the above
Correct Answer:
Verified
Q23: Proteins expressed in bacterial cells _.
I. fold
Q24: Engineered proteins can be expressed in a
Q25: Using site-directed mutagenesis, the mutation of a
Q26: It is common, when making mutations in
Q27: Adding restriction sites when constructing plasmids will
Q29: PCR-based mutagenesis is a widely used technique
Q30: Mutation of a serine residue to a(n)
Q31: _ suppression is used to incorporate unnatural
Q32: Subtilisin is a bacterial serine protease that
Q33: The green fluorescent protein (GFP) _.
I. is
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