How much DNA would be contained in 200 ?g of a sample that has an absorbance at 260nm of 0.40 and an absorbance at 280nm of 0.20?
A) 4 ?g
B) 6 ?g
C) 8 ?g
D) 10 ?g

Question

Quizplus · Accepted Answer

The absorbance at 260 nm is used to estimate DNA concentration. An absorbance of 1.0 at 260 nm corresponds to approximately 50 ?g/mL of DNA. Therefore, 0.40 absorbance corresponds to 20 ?g/mL. Since the sample is 200 ?g, the DNA content is 4 ?g.

How Much DNA Would Be Contained in 200 ?G of a Sample