Design a Southern blot experiment to check a transgenic mouse's DNA for integration of the GFP cDNA under control of a constitutive promoter. You many assume any array of restriction sites you wish in the target mouse chromosome and in the GFP transgene.
(a) Show sample results for a successful and an unsuccessful integration.
(b) Will mice from different founder lineages have the same site of integration?
(c) Describe techniques you would use to analyze the transgenic mice for transcription of the transgene and translation of the transgene.

Question

Design a Southern blot experiment to check a transgenic mouse's DNA for integration of the GFP cDNA under control of a constitutive promoter. You many assume any array of restriction sites you wish in the target mouse chromosome and in the GFP transgene. 
(a) Show sample results for a successful and an unsuccessful integration.
(b) Will mice from different founder lineages have the same site of integration?
(c) Describe techniques you would use to analyze the transgenic mice for transcription of the transgene and translation of the transgene.

Quizplus · Accepted Answer

The Answer of Design a Southern blot experiment to check a transgenic mouse's DNA for integration of the GFP cDNA under control of a constitutive promoter. You many assume any array of restriction sites you wish in the target mouse chromosome and in the GFP transgene. 
(a) Show sample results for a successful and an unsuccessful integration.
(b) Will mice from different founder lineages have the same site of integration?
(c) Describe techniques you would use to analyze the transgenic mice for transcription of the transgene and translation of the transgene.

Design a Southern Blot Experiment to Check a Transgenic Mouse's