Describe a toeprint assay involving mammalian ribosomal subunits and a mRNA in a cell-free extract that contains all the factors necessary for translation.
(a) What results would you expect to see with 40S ribosomal subunits alone?
(b) With 60S subunits alone?
(c) With both subunits and all amino acids except serine, which is required in the 15^th position of the polypeptide?

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The Answer of Describe a toeprint assay involving mammalian ribosomal subunits and a mRNA in a cell-free extract that contains all the factors necessary for translation. (a) What results would you expect to see with 40S ribosomal subunits alone? (b) With 60S subunits alone? (c) With both subunits and all amino acids except serine, which is required in the 15^th position of the polypeptide?

Describe a Toeprint Assay Involving Mammalian Ribosomal Subunits and a MRNA