You have discovered a minor class of rare introns that use a distinct splicing machinery involving snRNPs U110, U120, and U140. An experiment was performed to determine the order of assembly. A pre-mRNA was attached to agarose beads and incubated with purified splicing components. At time points from 0-20 minutes, RNA was extracted from the beads and analyzed by Northern blot with labeled probes specific for each RNA. In what order are the snRNPs assembled?

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The Answer of You have discovered a minor class of rare introns that use a distinct splicing machinery involving snRNPs U110, U120, and U140. An experiment was performed to determine the order of assembly. A pre-mRNA was attached to agarose beads and incubated with purified splicing components. At time points from 0-20 minutes, RNA was extracted from the beads and analyzed by Northern blot with labeled probes specific for each RNA. In what order are the snRNPs assembled?

You Have Discovered a Minor Class of Rare Introns That