Dideoxy DNA sequencing is based on chain termination with dideoxynucleotides. Why are normal deoxynucleotides also included in the reaction?
A) to act as a primer for chain termination by the dideoxynucleotides
B) to provide a template for DNA polymerase
C) to allow production of a range of synthesis products of different lengths.
D) to provide a 2?-OH required for incorporation of the dideoxynucleotides
Correct Answer:
Verified
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