Which of the following statements regarding 454 pyrosequencing is not true?
A) Each DNA fragment to be sequenced must be clonally amplified.
B) Dideoxy nucleotides are used to reversibly terminate chain elongation.
C) Nucleotide incorporation is detected as light generated by the luciferase enzyme.
D) Many sequencing reactions are carried in in parallel in a multi-well plate.
Correct Answer:
Verified
Q38: Dideoxy DNA sequencing is based on chain
Q39: It is common to use ddNTPs (dideoxynucleoside
Q40: The smallest fragment at the end of
Q41: The sequence read from a DNA sequencing
Q42: Automated DNA sequencing differs from manual DNA
Q44: Pulse-field electrophoresis is useful for separating
A) small
Q45: To read an old-fashioned Sanger sequencing gel
Q46: The polymerase chain reaction does not require
A)
Q47: Define the term "restriction endonuclease" and explain
Q48: Describe some of the key experiments and
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