Suppose You Are Setting Up an Assay in Which Patient

Suppose you are setting up an assay in which patient samples are run in duplicate.All duplicate patient results match except for one.Which of the following would be a reasonable explanation (or explanations) for the lack of reproducibility for the patient results?

A) The pipet tip for one of the patient analyses was not placed tightly on the micropipet.
B) The patient sample was "wicked" out when wiping the pipet tip.
C) The technologist did not depress the piston of the micropipet to the second stop when delivering patient sample in one of the analyses.
D) All of the above