Suppose there is the need to separate the components of a mixture in which the components are known to have a molecular weight of less than 1000 D, not be ionizable, and be relatively nonpolar with limited hydrogen-bonding potential.Which chromatographic method should be selected?

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Quizplus · Accepted Answer

Gel-permeation chromatography should not be considered for molecules with a molecular weight of less than 1000 D.Likewise, ion-exchange chromatography would not be the method of choice, since the molecules of interest are not ionizable.In addition, adsorption chromatography should be ruled out because of the importance of hydrogen bonding in this technique.The technique of choice is partition chromatography, which is more suitable for relatively nonpolar compounds.

Suppose There Is the Need to Separate the Components of a Mixture