Which of the following provides the correct explanation for a decrease in light scatter as concentration of hapten (such as a drug) increases in nephelometric inhibition immunoassay (NINIA) ?
A) As the concentration of hapten (drug) increases, the excess of antibody decreases, resulting in less light scatter.
B) As the concentration of hapten (drug) increases, more soluble hapten (drug) -antibody complexes form, reducing the amount of light scatter.
C) As the concentration of hapten (drug) increases, more hapten (drug) -antibody complexes form, which fix complement and destroy the complex, reducing the amount of light scatter.
D) As the concentration of hapten (drug) increases, more insoluble hapten (drug) -antibody complexes form, resulting in less light scatter.
Correct Answer:
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