Suppose your laboratory uses a noncompetitive labeled-antibody immunometric technique that incorporates spectrophotometry for the measurement of a particular antigen.As the amount of measured antigen present in the patient sample ________, the absorbance of the test solution will ________.

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In this assay technique, once the initial antibody is immobilized, the patient sample containing the antigen is added.Once the excess antigen is washed away, a labeled antibody is added.The more antigen in the patient sample, the more binding to the immobilized antibody in the initial step.This will result in more binding of the second antibody which is labeled.With increased binding of labeled antibody, there will be greater absorbance during measurement.The opposite is also true, where a decrease in the amount of antigen in the patient sample will result in a decrease in absorbance during measurement.

Suppose Your Laboratory Uses a Noncompetitive Labeled-Antibody Immunometric Technique That