In competitive-binding assays, the relationship between concentration of unlabeled ligand and percent labeled ligand bound can be described in which of the following ways?

Question

Quizplus · Accepted Answer

The relationship between the concentration of unlabeled ligand and the percent of labeled ligand bound in competitive-binding assays is logarithmic and inverse. As the concentration of the unlabeled ligand increases, it competes more effectively with the labeled ligand for binding sites, thereby decreasing the percentage of labeled ligand that is bound. This relationship is typically represented on a logarithmic scale to more clearly visualize changes, especially at lower concentrations of unlabeled ligand.

In Competitive-Binding Assays, the Relationship Between Concentration of Unlabeled Ligand