In the fluorescence polarization immunoassay (FPIA) technique, the amount of polarized fluorescent light detected when the fluorophore is excited with polarized light is higher for:

Question

Quizplus · Accepted Answer

Low-molecular-weight fluorescence-labeled ligands rotate freely in solution and when the label is excited, the fluorescence emission is depolarized.Large molecules such as the fluorescence-labeled ligand bound to antibody rotate more slowly than labeled ligands alone, resulting in an increased emission of polarized fluorescent light.

In the Fluorescence Polarization Immunoassay (FPIA) Technique, the Amount of Polarized