In microparticle-based light-scattering inhibition immunoassays, as concentration of measured ligand in the patient sample increases, the amount of light scattered in nephelometric measurements ______, and the absorbance reading in turbidimetric measurements ______.

Question

Quizplus · Accepted Answer

In this inhibition assay, the presence of ligand in the patient sample inhibits the rate of aggregation by competing with the agglutinator for antibody binding sites.Thus as concentration of ligand in the patient sample increases, agglutination decreases, resulting in a decrease in light scatter and absorbance readings.

In Microparticle-Based Light-Scattering Inhibition Immunoassays, as Concentration of Measured Ligand