Competitive-binding assays that require the antibody-bound, labeled ligand be physically separated from the free labeled ligand are called ________ techniques.Those techniques that do not require physical separation are called _______ techniques, and these assays require _______.

Question

Quizplus · Accepted Answer

Heterogeneous assays do require a step to separate antibody-bound, labeled ligand from free labeled ligand.Homogeneous assays do not require the separation step.To bypass this separation step, homogeneous assays require a more complex immunochemical reaction where one aspect of the reaction (e.g., the ligand-antibody binding) activates or inhibits an associated reaction.This type of close reliance on the previous step may continue for one or two additional steps, resulting in a complex immunochemical reaction.

Competitive-Binding Assays That Require the Antibody-Bound, Labeled Ligand Be Physically