Suppose your laboratory uses the ELISA configuration in which the antibody has been immobilized onto the solid phase.How would the result of the assay for a patient whose specimen is in a specific well be affected by improperly washing the solid phase of that particular well?

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Quizplus · Accepted Answer

In this technique, the patient sample containing the antibody of interest is added to a well coated with ligand for that antibody.Following incubation, the well is rinsed with water or buffer to remove any unbound antibody.Antihuman antibody conjugated with an indicator is then added to the well to react with antibody present in the well.If unbound antibody was not properly removed by the washing step, it would react along with the bound antibody, producing a falsely elevated result.

Suppose Your Laboratory Uses the ELISA Configuration in Which the Antibody