The two methods used in the clinical laboratory to determine osmolality are:

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Freezing-point depression and vapor-pressure depression are the two methods utilized in the clinical laboratory to measure osmolality.Freezing-point depression is the most common.It is not possible to measure osmotic pressure directly, since there is no membrane that is permeable to water but impermeable to all solutes.Likewise, boiling-point elevation is not suitable for clinical laboratory use, because boiling would cause proteins to coagulate.

The Two Methods Used in the Clinical Laboratory to Determine