What substrate concentration is typically utilized in enzymatic analyses to ensure zero-order kinetics?

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Quizplus · Accepted Answer

Use of the Michaelis-Menten equation defines the Michaelis-Menten constant Km as the substrate concentration necessary to produce a reaction velocity of ½ maximum velocity.Use of the Michaelis-Menten equation also shows that when substrate concentration is set at 10 × Km, the velocity is 91% of the maximum velocity for the enzymatic reaction.Thus to achieve maximum velocity and zero-order reaction conditions long enough for enzymatic analysis, the substrate concentration should be set at greater than 10 × Km, preferably closer to 100 × Km.

What Substrate Concentration Is Typically Utilized in Enzymatic Analyses to Ensure