Which of the following is necessary for maximal enzyme velocity?

Question

Quizplus · Accepted Answer

To measure the maximum velocity of an enzymatic reaction, substrate concentration must be in excess of enzyme concentration.Because the total number of active binding sites on the enzyme are limited and the amount of enzyme is constant, as the amount of substrate increases, the sites on the enzyme will become increasingly saturated.Beyond this point, with further increases in substrate concentration, as soon as the enzyme converts one substrate molecule to product, another substrate molecule will be available to take its place.At this level of substrate excess, the reaction becomes independent of substrate concentration, and the enzymatic reaction will proceed at its maximal velocity.

Which of the Following Is Necessary for Maximal Enzyme Velocity