Which of the following techniques would be useful for the correction of protein buildup on a spectrophotometer flow cell in an automated analyzer?

Question

Quizplus · Accepted Answer

Two-point kinetic analysis involves two absorbance readings followed by taking the difference in absorbance reading.Since protein buildup will effect both readings equally, calculating the change in absorbance will negate this interference.Bichromatic analysis involves measuring absorbance at two wavelengths, one at the wavelength of maximum absorption and the second at a wavelength at which the compound of interest does not absorb.In this technique, the protein buildup will scatter light at both wavelengths, allowing the absorbance resulting from the protein buildup to be negated.

Which of the Following Techniques Would Be Useful for the Correction