Given the following data for bichromatic analysis in which the analytical wavelength is 510 nm, determine the concentration of analyte in the patient sample.
Patient sample absorbance at 510 nm = 0.715
Patient sample absorbance at 550 nm = 0.113
Standard absorbance at 510 nm = 0.500
Standard absorbance at 550 nm = 0.113
Standard concentration = 25 mg/L

Question

Quizplus · Accepted Answer

The corrected absorbance for the patient sample is 0.602 (0.715 - 0.113).The corrected absorbance for the standard is 0.1125 (0.500 - 0.113).Given that the concentration of the standard is known as 25 mg/L, the corrected absorbances will be used with the following equation:
Cᵤ = Aᵤ/As × Cs = 0.602/0.1125 × 25 mg/L = 134 mg/L

Given the Following Data for Bichromatic Analysis in Which the Analytical