Suppose the result for a particular analyte by HPLC was much higher than anticipated, and the patient's physician questioned the result.Which of the following analytical modifications would be most useful in investigating the reason for the unexpectedly high patient result?

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Quizplus · Accepted Answer

In chromatography, the assumption is generally made that the analyte of interest is completely separated from other compounds.However, there is always the possibility of co-eluting compounds.When higher than expected results are obtained by chromatography, the presence of co-eluting compounds should always be considered.One technique that may be useful to identify the presence of co-eluting compounds is the measurement of absorption patterns at multiple wavelengths, simultaneously using a photo diode array (PDA) detector.The probability that the co-eluting compound will have the same spectral characteristics as the compound of interest at all wavelengths monitored is quite small.

Suppose the Result for a Particular Analyte by HPLC Was