The dideoxy sequencing method uses ddNTPs, which terminate DNA synthesis at specific bases.
-In the dideoxy sequencing reaction, what terminates DNA synthesis at a particular base?
A) The absence of a base on the ddNTP halts the DNA polymerase.
B) The ddNTP causes a break in the sugar-phosphate backbone.
C) DNA polymerase will not incorporate a ddNTP into the growing DNA strand.
D) The absence of a 3'-OH group on the ddNTP prevents the addition of another nucleotide

Question

Quizplus · Accepted Answer

The absence of a 3'-OH group on the ddNTP prevents the addition of another nucleotide, terminating DNA synthesis.

The Dideoxy Sequencing Method Uses DdNTPs, Which Terminate DNA Synthesis