Methotrexate is an inhibitor of which of the following enzymes?

Question

Quizplus · Accepted Answer

Methotrexate inhibits dihydrofolate reductase, an enzyme involved in the folate pathway, which is crucial for DNA synthesis and cell replication.  Tetrahydrofolate (THF) is regenerated from the dihydrofolate (DHF) product of the thymidylate synthase reaction by the action of dihydrofolate reductase (DHFR). Cells that are unable to regenerate THF suffer defective DNA synthesis and eventual death. Many anticancer drugs act directly to inhibit thymidylate synthase, or indirectly, by inhibiting DHFR. Many DHFR inhibitors have been synthesized, including methotrexate, aminopterin, and trimethoprim. Each of these is an analog of folic acid.

Methotrexate Is an Inhibitor of Which of the Following Enzymes