Using a cation-exchange resin,a mixture of 4 amino acids is separated using an elution gradient of increasing NaCl solution.What would be the correct elution sequence?
A)Arg,Asp,Lys,Ser
B)Lys,Arg,Asp,Ser
C)Asp,Ser,Lys,Arg
D)Ser,Asp,Lys,Arg

Question

Quizplus · Accepted Answer

Cation-exchange resins bind positively charged molecules more strongly. Amino acids with more positive charge (basic) will elute last in an increasing NaCl gradient. Asp is acidic, Ser is neutral, and Lys and Arg are basic, with Arg typically having a higher pI than Lys.

Using a Cation-Exchange Resin,a Mixture of 4 Amino Acids Is