Suppose a protein is treated with trypsin.Which of the following techniques could be used to determine its identity by peptide mass fingerprinting?
A)NMR
B)MALDI-TOF mass spectrometry
C)HPLC
D)gel electrophoresis

Question

Quizplus · Accepted Answer

MALDI-TOF mass spectrometry is a commonly used technique for peptide mass fingerprinting as it can accurately determine the mass-to-charge ratio of peptides in a sample. This allows for identification of the protein through comparison with a known protein database. NMR is not typically used for peptide mass fingerprinting, HPLC is used for peptide separation but not mass determination, and gel electrophoresis separates proteins based on size and charge, but does not provide information on the specific peptide sequences present in the sample.

Suppose a Protein Is Treated with Trypsin