Insulin is a polypeptide hormone that contains 2 short polypeptide chains linked by 2 interstrand disulfide bonds.Which of the following treatments would NOT be useful for determining its primary structure?
A)reduction of the peptides with SDS
B)sequencing of the peptides with Edman chemistry
C)separation of the peptides by chromatography techniques
D)alkylation of the peptides with iodoacetate

Question

Quizplus · Accepted Answer

Reduction of the peptides with SDS would break the disulfide bonds that hold the short polypeptide chains together, making it impossible to determine the primary structure of insulin. Sequencing the peptides with Edman chemistry, separation of the peptides by chromatography techniques, and alkylation of the peptides with iodoacetate are all useful methods for determining insulin's primary structure.

Insulin Is a Polypeptide Hormone That Contains 2 Short Polypeptide