Which of the following treatments is NOT a useful method for dissociating a heteromultimeric protein during sequencing?
A)high salt concentrations
B)6 N HCl at 110°C for 24 hours
C)8 M urea
D)6 M guanidinium chloride

Question

Quizplus · Accepted Answer

Treatment with 6 N HCl at 110°C for 24 hours is a very harsh method that can result in hydrolysis of the peptide bonds and destruction of the protein. It is not a useful method for dissociating a heteromultimeric protein during sequencing. The other listed methods, high salt concentrations (A), 8 M urea (C), and 6 M guanidinium chloride (D), are more commonly used and gentler methods that can effectively dissociate protein complexes.

Which of the Following Treatments Is NOT a Useful Method