When sequencing a protein,which of the following is an advantage of treating separate samples of the protein with 2 or more enzymes?
A)The products are homogeneous.
B)The products are sequenceable without further chromatography.
C)The products are fragments with the same N- and C-terminal amino acids.
D)The products are fragments with sequence overlaps.

Question

Quizplus · Accepted Answer

Treating separate samples of the protein with 2 or more enzymes generates fragments with different sizes and overlapping sequences. This allows for the generation of a longer contiguous sequence by piecing together the overlapping fragments. This process is known as shotgun sequencing and is commonly used in high-throughput sequencing methods.

When Sequencing a Protein,which of the Following Is an Advantage