Which of the following statements regarding enzymes and transition states is correct?
A)Stabilization of the transition state must be less than stabilization of ES for catalysis to occur.
B)Binding of substrate to an enzyme often causes strain,thus promoting transition-state formation.
C)The transition-state conformation of an enzyme-catalyzed reaction is identical to the conformation seen in the uncatalyzed transition state.
D)Formation of the transition state always assures that the reaction will proceed to product.

Question

Quizplus · Accepted Answer

Binding of substrate to an enzyme often involves inducing strain which promotes the formation of the transition state. This strain can cause the breaking of bonds in the substrate, leading to a higher energy intermediate that is closer to the transition state. This can lower the activation energy required for the reaction to proceed. The other statements are incorrect. Stabilization of the transition state must be greater than stabilization of ES for catalysis to occur (A). The transition-state conformation in enzyme-catalyzed reactions is NOT identical to the conformation seen in the uncatalyzed transition state (C). Formation of the transition state is necessary, but not sufficient for a reaction to proceed to product (D).

Which of the Following Statements Regarding Enzymes and Transition States